Protein Engineering

Engineering a Bifunctional Fusion Purine/Pyrimidine Nucleoside Phosphorylase for the Production of Nucleoside Analogs
Nucleoside phosphorylases (NPs) are pivotal enzymes in the salvage pathway, catalyzing the reversible phosphorolysis of nucleosides to …
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Rapid discovery of high-affinity antibodies via massively parallel sequencing, ribosome display and affinity screening
Developing therapeutic antibodies is laborious and costly. Here we report a method for antibody discovery that leverages the Illumina …
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Curse and Blessing of Non-proteinogenic Parts in Computational Enzyme Engineering
Enzyme engineering aims to improve or install a new function in biocatalysts for applications ranging from chemical synthesis to …
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New chemistries and enzymes for synthetic genetics
Beyond the natural nucleic acids DNA and RNA, nucleic acid chemistry has unlocked a whole universe of modifications to their canonical …
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Approaching boiling point stability of an alcohol dehydrogenase through computationally-guided enzyme engineering
Enzyme instability is an important limitation for the investigation and application of enzymes. Therefore, methods to rapidly and …
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Exploring the Biocatalytic Potential of a Self‐Sufficient Cytochrome P450 from Thermothelomyces thermophila
Among nature’s arsenal of oxidative enzymes, cytochrome P450s (CYPs) catalyze the most challenging reactions, the hydroxylations …
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Stabilization of cyclohexanone monooxygenase by computational and experimental library design
Enzymes often by far exceed the activity, selectivity, and sustainability achieved with chemical catalysts. One of the main reasons for …
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Switching the post-translational modification of translation elongation factor EF-P
Pollution, accidents, and misinformation have earned the pharmaceutical and chemical industry a poor public reputation, despite their …
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Experimental protocols for generating focused mutant libraries and screening for thermostable proteins
Many proteins are rapidly deactivated when exposed to high or even ambient temperatures. This cannot only impede the study of a …
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Manipulating the stereoselectivity of the thermostable Baeyer–Villiger monooxygenase TmCHMO by directed evolution
Baeyer–Villiger monooxygenases (BVMOs) and evolved mutants have been shown to be excellent biocatalysts in many stereoselective …
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